Global Deglycosylation in High Throughput LC-MS Glycomics: Comparing Microwave, Pressure, and Ultrasound Effects on PNGase F with Human Serum
Conclusion
• Head-to-head comparisons of enzyme reactors were performed under controlled conditions.
• The high enzyme concentration set yielded comparable PNGase F activity in each reactor tested.
• The low enzyme concentration conditions demonstrated each reactor method provided an enhancement of enzymatic activity relative to the control
• Follow-up experiments will be designed to produce optimal glycan signatures now that driving factors for enhancement of enzymatic activity have been identified.